Custom Neural Differentiation

In general, there are two types of culture systems for directed neural differentiation of hESC, including adherent and suspension culture. In a prolonged culture, an embryoid body (EB) can form a multilayered structure and cavities filled with cystic fluid. In addition, a variety of signaling molecules, factors, and conditioned media have been used for directed neural differentiation.

For example, stromal-derived inducing activity (SDIS) mediated neural differentiation presents an efficient method, but unknown stromal factors may be introduced.
Retinoic acid (RA) plays important role in neural development and activity, RA-mediated neural differentiation occurs by activation of genes, such as NFkB, SOX1, SOX6, BRN2, PSEN1, MAP2.
Bone Morphogenetic Protein (BMP) signaling promotes the generation of neuronal, and Fibroblast Growth Factor (FGF) signaling has been implicated in the induction and patterning of neural tissues.
The Neurobasal medium is essential for the derivation and maintenance of NP cells.